This chemical is widely used as a fluorescent dye in various biological and biochemical applications. It is particularly valuable in fluorescence microscopy, where it helps label and visualize specific molecules or structures within cells. Its high brightness and photostability make it suitable for long-term imaging studies. Additionally, it is employed in flow cytometry to detect and analyze cell populations based on fluorescence signals. The dye is also utilized in fluorescence resonance energy transfer (FRET) experiments to study molecular interactions and distances at a nanoscale level. Its compatibility with a range of biological samples and instruments further enhances its utility in research and diagnostics.