TMRM is widely used in biological research as a fluorescent dye to assess mitochondrial membrane potential in live cells. Its ability to accumulate in active mitochondria makes it a valuable tool for studying cellular health, apoptosis, and mitochondrial dysfunction. Researchers often employ TMRM in conjunction with fluorescence microscopy or flow cytometry to visualize and quantify mitochondrial activity. Additionally, it is used in neurobiology to investigate neuronal energy metabolism and in cancer research to study the role of mitochondria in tumor cell survival. Its low toxicity and high sensitivity make it a preferred choice for real-time monitoring of mitochondrial dynamics in various experimental models.