This chemical is widely used as a pH-sensitive fluorescent dye in biological and biochemical research. It is particularly valuable for measuring intracellular pH levels in live cells due to its dual-emission properties, which allow for ratiometric measurements. This feature enhances accuracy by minimizing artifacts caused by variations in dye concentration, cell thickness, or photobleaching. It is commonly employed in fluorescence microscopy, flow cytometry, and spectroscopy to study pH dynamics in various cellular processes, such as metabolic activity, organelle function, and cellular responses to environmental changes. Its compatibility with a range of cell types and its ability to provide precise pH measurements make it a crucial tool in cell biology and biomedical research.