Dihydrorhodamine 6G is widely used as a fluorescent probe in biological and chemical research. It is particularly valuable in detecting reactive oxygen species (ROS) within cells, making it a crucial tool in studying oxidative stress and related cellular processes. The compound’s ability to fluoresce upon oxidation allows researchers to visualize and quantify ROS production in live cells or tissues. It is also employed in assays to evaluate the antioxidant capacity of various compounds, helping to understand their protective effects against oxidative damage. Additionally, its application extends to monitoring enzymatic activities and studying mitochondrial function, as ROS generation is often linked to these processes. Its sensitivity and specificity make it a preferred choice in biomedical and environmental studies.