H-1152 is primarily used in biochemical research as a potent and selective inhibitor of Rho-associated protein kinase (ROCK). Its application is significant in studying cellular processes such as cytoskeleton organization, cell migration, and smooth muscle contraction. Researchers utilize H-1152 to investigate the role of ROCK signaling in various physiological and pathological conditions, including cardiovascular diseases, cancer, and neurological disorders. Its high specificity makes it a valuable tool for elucidating the mechanisms of ROCK-mediated pathways and for developing potential therapeutic strategies targeting these pathways.