This compound is primarily used in research settings to study the role of Rho kinase (ROCK) signaling pathways in various biological processes. It is a potent and selective inhibitor of Rho kinase, making it valuable for investigating cellular mechanisms such as cytoskeletal organization, cell migration, and smooth muscle contraction. Researchers utilize it to explore its effects on conditions like hypertension, cardiovascular diseases, and neurological disorders, where Rho kinase activity is implicated. Additionally, it is employed in studies examining endothelial function, inflammation, and cancer cell behavior, providing insights into potential therapeutic targets. Its high selectivity and efficacy make it a critical tool in pharmacological and biochemical research.