H-1152 dihydrochloride is primarily used in research settings to study cellular processes, particularly those involving the Rho-associated protein kinase (ROCK) signaling pathway. It acts as a potent and selective inhibitor of ROCK, making it a valuable tool for investigating the role of this pathway in various biological functions. Researchers utilize it to explore its effects on cytoskeleton organization, cell motility, and contractility, which are critical in understanding conditions like cancer metastasis, cardiovascular diseases, and neurological disorders. Additionally, it is employed in studies focused on vascular smooth muscle contraction and endothelial function, providing insights into hypertension and other vascular-related diseases. Its application extends to in vitro and in vivo experiments, aiding in the development of potential therapeutic strategies targeting the ROCK pathway.