ROCK inhibitor-2 is primarily used in biomedical research to study cellular processes involving the Rho-associated protein kinase (ROCK) pathway. It is applied to investigate the role of ROCK in cell migration, cytoskeletal organization, and cell contraction, which are critical in understanding diseases like cancer, cardiovascular disorders, and neurological conditions. Researchers use it to inhibit ROCK activity, allowing them to explore its effects on cell behavior and signaling mechanisms. Additionally, it is utilized in drug development studies to evaluate potential therapeutic agents targeting ROCK-related pathways. Its application extends to in vitro and in vivo models to assess its efficacy in modulating cellular responses and disease progression.