ROCK-IN-1 is primarily used in biochemical and pharmacological research as a potent and selective inhibitor of Rho-associated protein kinase (ROCK). Its application is significant in studying cellular processes such as cell migration, cytoskeletal reorganization, and smooth muscle contraction. Researchers utilize it to investigate the role of ROCK signaling pathways in various diseases, including cardiovascular disorders, cancer, and neurological conditions. Additionally, it serves as a valuable tool in experimental models to explore potential therapeutic strategies targeting ROCK-mediated mechanisms. Its specificity and effectiveness make it a key compound in advancing understanding of cellular dynamics and disease pathology.