ROCK2-IN-2 is primarily utilized in biomedical research as a selective inhibitor targeting the Rho-associated protein kinase 2 (ROCK2). Its application is significant in studying cellular processes such as cytoskeletal organization, cell motility, and smooth muscle contraction. Researchers employ this compound to investigate its potential therapeutic effects in diseases where ROCK2 activity is dysregulated, including cancer, cardiovascular disorders, and neurological conditions. Additionally, it serves as a valuable tool in pharmacological studies to understand the role of ROCK2 signaling pathways in various physiological and pathological contexts.