One-step PCR Mix is widely used in molecular biology for applications that require reverse transcription and polymerase chain reaction (PCR) in a single tube. It simplifies workflows by combining cDNA synthesis and DNA amplification, making it ideal for gene expression analysis, pathogen detection, and RNA virus studies. This mix is particularly useful in diagnostics, where rapid and sensitive detection of RNA targets is essential, such as in COVID-19 testing. It also supports high-throughput screening and reduces the risk of contamination by minimizing manual handling. Researchers often use it for cloning, mutagenesis, and quantitative PCR (qPCR) experiments, ensuring efficiency and reproducibility in RNA-based studies.