Tris-Tricine-SDS Running Buffer, Anode, is primarily used in polyacrylamide gel electrophoresis (PAGE) for the separation and analysis of low molecular weight proteins and peptides. It is particularly effective in resolving small proteins (typically below 30 kDa) that are difficult to separate using traditional SDS-PAGE buffers. The buffer system provides a stable pH environment and enhances the resolution of protein bands, making it a preferred choice for detailed protein studies. It is commonly applied in biochemical research, proteomics, and molecular biology to analyze protein composition, purity, and molecular weight distribution. Additionally, it is used in the characterization of membrane proteins and other complex protein mixtures, aiding in the identification and study of protein structures and functions.