Tris-Tricine-SDS Running Buffer, Cathode, is primarily used in electrophoresis, specifically for the separation of low molecular weight proteins and peptides. This buffer system is particularly effective in resolving proteins in the range of 1 to 100 kDa, making it ideal for analyzing small proteins that are difficult to separate using traditional SDS-PAGE. The cathode buffer works in conjunction with the anode buffer to create a stable pH gradient, ensuring sharp and clear protein bands. It is commonly employed in research laboratories for protein characterization, quality control in protein production, and in studies involving proteomics. Its ability to provide high-resolution separation makes it a valuable tool in biochemical and molecular biology research.