Tris-Acetate Buffer is widely used in molecular biology, particularly in electrophoresis techniques. It is commonly employed as a running buffer in agarose gel electrophoresis for the separation and analysis of DNA fragments. The buffer provides a stable pH environment, ensuring optimal DNA migration and resolution during the process. Additionally, Tris-Acetate Buffer is utilized in protein electrophoresis, where it helps maintain the integrity and separation of proteins based on their size and charge. Its compatibility with various staining and detection methods further enhances its utility in laboratory applications.