Tris-Glycine-SDS PAGE Running Buffer (10X concentrate) is a standard buffer used in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to separate denatured proteins primarily based on their molecular weight. The inclusion of SDS ensures protein denaturation and uniform negative charge, allowing size-based separation. It is widely employed in research and diagnostic laboratories for protein analysis, purity assessment, molecular weight determination, and Western blotting applications. This buffer is typically diluted to 1X (25 mM Tris, 192 mM Glycine, 0.1% SDS, pH 8.3) before use in gel running.