Tricine is widely used as a buffer in biochemical and molecular biology research. It is particularly effective in maintaining stable pH conditions in the range of 7.4 to 8.8, making it suitable for various laboratory applications. One of its primary uses is in electrophoresis, especially for separating small proteins and peptides in polyacrylamide gels. Tricine-SDS-PAGE is a common technique that provides better resolution for proteins with molecular weights below 30 kDa compared to traditional glycine-based systems. Additionally, Tricine is utilized in enzyme assays and protein purification processes, where its low ultraviolet absorbance minimizes interference with spectrophotometric measurements. Its compatibility with biological systems also makes it a preferred choice for cell culture media and other biochemical studies requiring precise pH control.