Used in analytical chemistry for the determination of metal ions. In colorimetric methods, it forms colored complexes with ions such as iron (red Fe(SCN)₃), cobalt, and copper. It is also employed in precipitation titrations, such as the Mohr or Fajans methods, for quantifying silver (Ag⁺) and mercury (Hg²⁺), where thiocyanate ions form sparingly soluble white precipitates to indicate the endpoint. Commonly applied in environmental testing (e.g., water quality analysis), industrial quality control, and research laboratories to calibrate instruments and validate test procedures. Additionally, utilized in the preparation of reagents for clinical diagnostics and in the assessment of thiocyanate levels in biological samples. Its standardized concentration ensures accurate and reproducible results in titrations and spectrophotometric analyses.