This buffer is primarily used in protein electrophoresis to prepare samples for separation on polyacrylamide gels. It helps denature proteins, ensuring they unfold and maintain a consistent charge-to-mass ratio, which is crucial for accurate migration during electrophoresis. The inclusion of LDS (Lithium Dodecyl Sulfate) aids in solubilizing proteins and enhancing their separation. Tris-acetate provides the appropriate pH environment for optimal protein stability and gel performance. This buffer is particularly useful for loading samples into precast gels or custom gels in SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) applications, making it a key reagent in molecular biology and biochemistry research for analyzing protein composition, purity, and molecular weight.