Azomethine H is widely used as a reagent in analytical chemistry for the spectrophotometric determination of various metal ions. It is particularly effective in detecting and quantifying trace amounts of metals like copper, nickel, and cobalt in environmental and industrial samples. The compound forms colored complexes with these metals, allowing for precise measurement through UV-Vis spectroscopy. Additionally, Azomethine H is employed in biochemical research for studying metal-binding interactions and enzyme activities. Its sensitivity and selectivity make it a valuable tool in quality control processes, ensuring the safety and compliance of water, food, and pharmaceutical products.