Used primarily in microbiology for the selective isolation and identification of Vibrio species, particularly Vibrio cholerae and Vibrio parahaemolyticus. The medium supports the growth of these pathogens while inhibiting other bacteria, making it valuable in clinical and environmental testing. It is commonly employed in water quality analysis, food safety monitoring, and outbreak investigations to detect contamination. The inclusion of thiosulfate and citrate aids in differentiating Vibrio species based on their ability to metabolize sucrose, while bile salts suppress non-target organisms. This medium is essential in public health laboratories for ensuring rapid and accurate detection of waterborne and foodborne pathogens.