Used in microbiology laboratories to isolate and differentiate Clostridium species, particularly from clinical or environmental samples. Supports the growth of anaerobic bacteria and helps distinguish between pathogenic and non-pathogenic strains based on colony morphology and biochemical reactions. Commonly used in diagnostic settings to identify Clostridium difficile and other spore-forming anaerobes associated with gastrointestinal infections. Also applied in food and pharmaceutical industries to test for anaerobic contamination.