Used primarily in microbiology for the selective isolation and differentiation of Clostridium species, especially Clostridium perfringens, from clinical and non-clinical samples. The medium supports the growth of anaerobic bacteria while inhibiting contaminants through selective agents. Colonies of Clostridium perfringens typically exhibit a distinctive double zone hemolysis on blood-enriched versions, allowing for easy identification. Commonly applied in food safety testing, veterinary diagnostics, and environmental monitoring to detect clostridia in samples like feces, soil, and food products. Its reinforced formulation enhances recovery of injured or stressed organisms, making it reliable for samples with low viability.