Used in microbiology for the detection and enumeration of coliform bacteria, especially Escherichia coli, in water, food, and environmental samples. The medium supports the growth of coliforms while inhibiting non-target bacteria due to selective agents. When combined with LES (Lauryl Tryptose Broth with EC), it helps confirm thermotolerant coliforms through fermentation of lactose and production of gas and acid. m-Endo Agar is commonly used in membrane filtration tests, where bacterial colonies exhibit characteristic red or pink color with a metallic sheen, aiding in quick identification. It is widely applied in drinking water and wastewater testing to ensure compliance with public health standards.