ML 154 is primarily used in research settings as a selective antagonist for serotonin receptors, particularly targeting the 5-HT2C receptor subtype. It is employed to study the role of serotonin signaling in neurological and psychiatric conditions such as depression, anxiety, and schizophrenia. Due to its selectivity, ML 154 helps scientists differentiate the effects mediated by 5-HT2C from other serotonin receptor subtypes, making it a valuable tool in neuropharmacology. It is also used in behavioral studies to investigate appetite regulation, mood, and impulsivity, where serotonin pathways are involved. Its application remains confined to preclinical research and is not used in clinical or therapeutic contexts.