WAY-620220 is primarily used in neuroscience research as a potent and selective antagonist for the 5-HT6 receptor subtype. Its main application lies in studying the role of serotonin signaling in cognitive functions, particularly in models of learning and memory. Due to its ability to cross the blood-brain barrier effectively, it is employed in preclinical studies to evaluate potential treatments for neurodegenerative disorders such as Alzheimer's disease. Researchers also use it to investigate the impact of 5-HT6 receptor modulation on behaviors related to anxiety, depression, and schizophrenia. Its selectivity makes it a valuable tool for distinguishing 5-HT6-mediated effects from those of other serotonin receptors in pharmacological assays.