WAY-620220 is primarily used in neuroscience research as a potent and selective antagonist for the 5-HT6 receptor subtype. Its main application lies in studying the role of serotonin signaling in cognitive functions, particularly in models related to learning and memory. Due to its high selectivity, it is employed to investigate potential therapeutic pathways for neurodegenerative disorders such as Alzheimer's disease. Researchers use WAY-620220 to modulate 5-HT6 receptor activity in preclinical models, helping to clarify the receptor's involvement in behavioral and cognitive processes. It is also utilized in pharmacological studies to assess the effects of 5-HT6 receptor blockade on neurotransmitter release, especially acetylcholine and glutamate, in specific brain regions. Its application remains confined to laboratory and experimental settings, with no current clinical use in humans.