WAY-313165 is primarily used in neuroscience research as a potent and selective antagonist of the 5-HT6 receptor. Its main application lies in studying the role of serotonin signaling in cognitive functions, memory, and learning processes. Due to its ability to cross the blood-brain barrier effectively, it is utilized in preclinical models to investigate potential treatments for neurodegenerative disorders such as Alzheimer's disease. It has also been explored for its effects on behavioral models related to depression, anxiety, and schizophrenia, helping researchers understand the therapeutic potential of modulating 5-HT6 receptor activity. Additionally, WAY-313165 serves as a pharmacological tool to validate target engagement in central nervous system drug development.