WAY-333449 is primarily used in scientific research to investigate serotonin receptor activity, particularly targeting the 5-HT6 receptor subtype. It serves as a selective antagonist, helping researchers understand the role of this receptor in cognitive function, memory, and neurodegenerative conditions such as Alzheimer's disease. Due to its high selectivity, it is valuable in pharmacological studies aiming to dissect signaling pathways and behavioral effects mediated by 5-HT6 receptors. It has also been employed in preclinical models to assess potential effects on learning and memory, making it a tool compound in the development of central nervous system therapeutics. Its use is restricted to laboratory and experimental settings, with no clinical applications approved to date.