WAY-620496 is primarily used in neuroscience research as a potent and selective antagonist for the 5-HT6 receptor, making it a valuable tool for studying serotonin signaling pathways in the brain. Its high selectivity allows researchers to investigate the role of 5-HT6 receptors in cognitive functions, memory formation, and neurodegenerative conditions such as Alzheimer's disease. It has also been explored for its potential in modulating behaviors related to depression, anxiety, and schizophrenia in preclinical models. Due to its ability to cross the blood-brain barrier effectively, it is frequently used in in vivo studies to assess central nervous system activity. Additionally, WAY-620496 serves as a reference compound in pharmacological assays aimed at evaluating new 5-HT6-targeting therapeutics.