WAY-325485 is primarily used in scientific research as a selective agonist for the 5-HT6 receptor subtype. It is employed to study the role of serotonin signaling in cognitive functions, particularly in models related to learning and memory. Due to its high selectivity, it helps researchers dissect the specific contributions of 5-HT6 receptors in neurochemical pathways without significant off-target effects. It has been investigated for potential therapeutic relevance in neurodegenerative disorders such as Alzheimer's disease, where modulation of serotonin receptors may improve cognitive deficits. Additionally, WAY-325485 is used in pharmacological studies to evaluate the effects of 5-HT6 activation on neurotransmitter release, especially acetylcholine and glutamate, in various brain regions. Its application remains confined to preclinical research and is not used in clinical or industrial settings.