NF-κB-Jurkat is widely used in biomedical research to study cellular signaling pathways related to inflammation, immune response, and cancer. It serves as a critical tool in assays that monitor NF-κB activation, a key transcription factor involved in regulating gene expression during immune and stress responses. The cell line is particularly valuable in drug discovery and toxicology studies, where researchers assess the efficacy or safety of new compounds by measuring their impact on NF-κB signaling. Its consistent and measurable response makes it ideal for high-throughput screening of anti-inflammatory and immunomodulatory agents. Additionally, it supports research into viral infection mechanisms, as many viruses exploit the NF-κB pathway to enhance replication.