huTNFR2-Jurkat is primarily used as a cellular assay system to study TNF receptor 2 (TNFR2) signaling in human immune cells. It serves as a reporter cell line for evaluating the activation of the NF-κB pathway downstream of TNFR2 engagement. This cell line is widely applied in immunology and drug development research to screen and characterize therapeutic agents targeting the TNF pathway, such as agonists or antagonists of TNFR2. It is particularly useful in assessing the functional activity of biologics, including monoclonal antibodies and recombinant TNF ligands. Due to its stable expression of human TNFR2 and integration of a reporter gene, it enables high-throughput screening and quantitative analysis of compounds modulating TNFR2-mediated responses, which are relevant in autoimmune diseases, cancer immunotherapy, and inflammatory conditions.