Sephadex G15 is widely used in biochemical and biotechnological laboratories for the separation and purification of small molecules and peptides (up to approximately 1,500 Da). Its application is particularly valuable in gel filtration chromatography, where it effectively separates molecules based on size exclusion. This makes it an essential tool for desalting, buffer exchange, and fractionation of small biomolecules. In purification workflows, Sephadex G15 is often employed to remove salts or other small contaminants from samples without altering their structure or function. Additionally, it is used in the preparation of samples for downstream analytical techniques such as mass spectrometry or electrophoresis. Its ability to handle small volumes and provide high-resolution separation makes it a preferred choice for research involving low molecular weight compounds.