Used primarily in gel filtration chromatography for the separation and purification of biomolecules, particularly small peptides and proteins. It is effective in desalting and buffer exchange processes, where it helps remove salts or small molecules from larger biomolecules. Also employed in the fractionation of polysaccharides and nucleic acids, providing a simple and efficient method for size-based separation. In biochemical research, it aids in sample preparation by isolating target molecules from complex mixtures. Additionally, it is utilized in the pharmaceutical industry for purifying drug compounds and analyzing molecular interactions. Its hydrophilic properties make it suitable for aqueous solutions, ensuring minimal interaction with analytes.