Sephadex LH-20 is widely used in the purification and separation of small molecules, particularly in the field of natural product chemistry. It is effective in isolating compounds such as flavonoids, steroids, and other organic molecules from complex mixtures. Its ability to function in both aqueous and organic solvents makes it versatile for various chromatographic applications, including size exclusion, adsorption, and partition chromatography. In pharmaceutical research, it aids in the separation and purification of drug compounds and their metabolites. Additionally, it is employed in the food industry for the purification of bioactive components from plant extracts. Its high resolution and compatibility with a wide range of solvents make it a valuable tool in analytical and preparative chromatography.