Sephadex G50 is widely used in biochemistry and molecular biology for size exclusion chromatography. It is particularly effective in separating molecules based on their size, making it a valuable tool for purifying proteins, peptides, and nucleic acids. In protein purification, it helps remove smaller contaminants or separate proteins of different molecular weights. It is also used in desalting processes, where it efficiently removes salts or other small molecules from larger biomolecules. Additionally, Sephadex G50 is employed in the preparation of samples for further analytical techniques, such as gel electrophoresis or mass spectrometry. Its stability and ease of use make it a popular choice in laboratory settings for fractionation and purification tasks.