Used in environmental and microbiological research to detect and quantify cytochrome cd1 nitrite reductase (Nirs) enzymes in soil samples. This ELISA kit enables scientists to assess denitrifying microbial activity, which is critical for understanding nitrogen cycling in ecosystems. It is particularly useful in studies related to soil fertility, greenhouse gas emissions (such as nitrous oxide), and environmental pollution. The kit supports rapid, sensitive, and specific measurement of Nirs protein levels, helping evaluate the functional potential of denitrifying bacteria in agricultural, forest, and contaminated soils.