Used in agricultural and environmental research to measure protease enzyme activity in soil samples. Helps assess soil health and microbial activity, as protease plays a key role in nitrogen cycling by breaking down organic proteins into absorbable nutrients. The ELISA method provides a sensitive and specific way to quantify protease levels, supporting studies on soil fertility, land management practices, and ecosystem productivity. Commonly applied in research related to sustainable farming, composting, and bioremediation projects.