Sphingosine (d14:1) is widely used in biochemical research to study cellular signaling pathways, particularly those involving sphingolipid metabolism. It serves as a key intermediate in the synthesis of complex sphingolipids, which are essential components of cell membranes and play critical roles in cell regulation, apoptosis, and signal transduction. Researchers utilize it to investigate the mechanisms of ceramide formation and its impact on cell survival and death. Additionally, it is employed in lipidomics studies to understand lipid dynamics in health and disease, including cancer, neurodegenerative disorders, and metabolic syndromes. Its application extends to the development of therapeutic strategies targeting sphingolipid-related pathways.