C6 NBD Sphingomyelin (d18:1/6:0) is widely used in biological research as a fluorescent lipid probe. Its primary application is in studying lipid dynamics, membrane organization, and intracellular trafficking. The NBD (nitrobenzoxadiazole) fluorophore attached to the sphingomyelin allows for real-time visualization and tracking of lipid movement within cellular membranes. Researchers use it to investigate lipid rafts, membrane fluidity, and the behavior of sphingolipids in various cellular processes. It is also employed in fluorescence microscopy and flow cytometry to monitor lipid distribution and metabolism in live cells. Additionally, it serves as a tool for examining the role of sphingomyelin in signaling pathways and membrane-associated diseases.