C6 NBD Ceramide (d18:1/6:0) is widely used in biological and biochemical research as a fluorescent lipid probe. Its primary application is in studying lipid metabolism, membrane dynamics, and intracellular trafficking. The fluorescent NBD (nitrobenzoxadiazole) tag allows for real-time visualization and tracking of ceramide movement within cells, making it a valuable tool for investigating lipid distribution and transport processes. It is particularly useful in fluorescence microscopy and flow cytometry experiments to analyze lipid behavior in live or fixed cells. Additionally, it aids in understanding the role of ceramides in cellular signaling, apoptosis, and membrane structure. Its application extends to research on lipid-related diseases, such as metabolic disorders and cancer, providing insights into lipid-mediated pathways.