NB-598 is primarily known for its role as a potent and selective inhibitor of squalene epoxidase, an enzyme involved in the cholesterol biosynthesis pathway. This property makes it a valuable tool in biochemical research, particularly in studies focused on understanding cholesterol metabolism and its regulation. By inhibiting squalene epoxidase, NB-598 effectively blocks the production of cholesterol, which is useful in investigating the physiological and pathological roles of cholesterol in various diseases, such as cardiovascular disorders and certain cancers. Additionally, it has been explored in the context of antifungal research, as cholesterol biosynthesis is also critical in fungal cell membrane integrity. Its application in these areas helps in the development of therapeutic strategies targeting cholesterol-related pathways or fungal infections.