Used primarily in microbiology to detect bacterial endospores, this staining technique helps differentiate spore-forming bacteria from non-spore-formers in clinical, environmental, and industrial samples. It is commonly applied in medical diagnostics to identify pathogens like Clostridium and Bacillus species, which can cause serious infections. In food safety and pharmaceutical manufacturing, the method ensures sterility by confirming the presence or absence of spores in products and on surfaces. The stain is also used in research settings to study spore formation, resistance mechanisms, and microbial survival under extreme conditions.