Pregnenolone monosulfate is primarily utilized in research settings to study its role as a neurosteroid and its effects on the central nervous system. It is often investigated for its potential impact on cognitive functions, memory enhancement, and neuroprotection. Researchers explore its interactions with neurotransmitter systems, particularly GABA and NMDA receptors, to understand its influence on brain activity and behavior. Additionally, it is used in studies related to stress response, mood regulation, and neurodegenerative diseases, such as Alzheimer's and Parkinson's. Its applications also extend to exploring its role in hormone synthesis pathways and its potential therapeutic effects in conditions involving hormonal imbalances.