This compound is primarily used in research and clinical studies as a deuterium-labeled analog of 25-Hydroxyvitamin D2. It serves as an internal standard in mass spectrometry and other analytical techniques to accurately measure and quantify levels of 25-Hydroxyvitamin D2 in biological samples. This is crucial for assessing vitamin D status in individuals, particularly in diagnosing deficiencies or monitoring treatment efficacy. Additionally, it aids in studying vitamin D metabolism and its role in various physiological processes, such as bone health, immune function, and calcium regulation. Its labeled structure ensures precise tracking and analysis without interfering with natural metabolic pathways.