Used primarily as a reference standard in steroid metabolism studies, this compound serves as a backbone structure for evaluating enzymatic activity in steroidogenic pathways. It is instrumental in research involving 5α-reductase, the enzyme responsible for converting testosterone to dihydrotestosterone. Due to its stable saturated ring system, it acts as an internal standard in gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) analyses for accurate quantification of androgenic steroids in biological samples. Its inert nature makes it suitable for use in pharmacokinetic and metabolic stability assays during drug development, particularly for compounds targeting hormonal disorders.