Digitoxigenin is primarily used in the field of pharmacology and medical research due to its role as a precursor in the synthesis of cardiac glycosides, such as digoxin and digitoxin. These compounds are crucial in the treatment of various heart conditions, including atrial fibrillation, atrial flutter, and heart failure. Digitoxigenin itself exhibits cardiotonic properties, which help in enhancing the force of heart contractions and improving cardiac output. Additionally, it is employed in biochemical studies to investigate the mechanisms of action of cardiac glycosides and their effects on sodium-potassium ATPase activity. Its application extends to the development of new therapeutic agents targeting cardiovascular diseases.