Di-8-ANEPPS is widely used as a fluorescent dye in biological research, particularly for studying cell membrane potentials. It is a voltage-sensitive dye that changes its fluorescence properties in response to alterations in membrane potential, making it a valuable tool in electrophysiology and neuroscience. Researchers use it to visualize and measure electrical activity in neurons, cardiac cells, and other excitable tissues. Its ability to provide real-time, non-invasive monitoring of membrane potential changes has made it essential for understanding cellular communication and function. Additionally, Di-8-ANEPPS is employed in imaging techniques to study the effects of drugs, toxins, or other stimuli on cellular activity. Its high sensitivity and compatibility with various imaging systems make it a preferred choice for dynamic studies of membrane physiology.