Used in biochemical research as an intermediate in glycolysis and gluconeogenesis studies. Serves as a substrate in enzyme assays involving triose phosphate isomerase and aldolase. Employed in metabolic pathway reconstructions and in vitro phosphorylation experiments. It also plays a key role in lipid biosynthesis, where it is converted to glycerol-3-phosphate, a precursor for triglycerides and phospholipids. Its magnesium complex enhances stability and solubility in aqueous reaction systems, making it suitable for cellular metabolism simulations and enzymatic synthesis applications, including in synthetic biology for biofuel and high-value chemical production.