N-Succinyl sulfanilamide sodium salt is primarily utilized in biochemical research as a substrate for studying enzyme activity, particularly in assays involving sulfonamide-related enzymes. It serves as a key compound in investigating the mechanisms of enzyme inhibition and the metabolic pathways of sulfonamides. Additionally, it is employed in the development of analytical methods for detecting and quantifying sulfonamide derivatives in various samples, contributing to advancements in pharmaceutical and environmental analysis. Its role in these applications aids in understanding drug interactions and optimizing therapeutic efficacy.